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to the manufacturer. Sigma- Reagent, Amount. Heparin (Sigma-Aldrich H3149, 10,000 units), 10 mg. caution PBS without Ca2+/Mg2+, 10 mL. Resuspend heparin in PBS and store aliquots Dissolve 5 g of solid heparin sodium (Sigma H3149-25KU) in 45 ml of sterile water. Sterile filter heparin solution through a 0.22 µm filter.

H3149 sigma

Real-Time Polymerase Chain Reaction RNA was purified using TRI Reagent Solution according to the manufacturer’sprotocol (ThermoFisher Scientific LifeSciences). HUDEP-2 cells were differentiated in a two-phase differentiation protocol consisting of IMDM supplemented with 5% human AB serum (H6914, Sigma), 10μg/mL recombinant human insulin (91077C, Sigma), 330μg/mL holo-transferrin (T4132, Sigma), 3 units/mL EPO (Amgen), 1μg/mL doxycycline (D9891, Sigma), 2 units/mL heparin (H3149, Sigma), and 1% penicillin/streptomycin. 50 ng/mL human SCF (300-07, Peprotech) was included in the phase one of the culture (day 1-3) and withdrawn in the phase two of Heparin (H3149, Sigma-Aldrich, St. Louis, MO, USA), 5% newborn calf serum (N4762, Sigma-Aldrich, St. Louis, MO, USA), 0.2 g/mL bovine serum albumin (BSA) (A9418, Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-glutamine (G7513, Sigma-Aldrich, St. Louis, MO, USA), 100 g/mL Streptomycin, and #T4132), 1% penicillin/streptomycin, 10ng/ml heparin (Sigma, H3149), 10ug/ml insulin (Sigma, Cat. #I9278), 1ug/ml doxycycline. Peripheral blood mononuclear cells (PBMCs) were obtained from the University of Pennsylvania Human Immunology Core.

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No. E2759, Sigma-Aldrich, St. Louis, MO, USA) were added to the EC-culture medium SIGMA HEPARIN SODIUM CELL CULTURE TESTED $ 110.00. HEPARIN SODIUM CELL CULTURE TESTED quantity.

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Add to cart. SKU: H3149-100KU Category: SIGMA.

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For further inquiries, please contact Technical Service. Sigma Aldrich H3149-50KU Heparin Sodium Salt Powder from Porcine Intestinal Mucosa, Grade 1-A, BioR (0) No Reviews yet.

50 ng/mL human SCF … 2021-01-21 2015-04-01 The papain was inactivated using ovomucoid solution (cat# 542000, SciQuest), tissue was briefly treated with DNase (cat# 10104159001, Sigma) and passed through the 100μm cell strainer (cat# 542000, Greiner Bio-One). Two cell culture conditions were initiated based on the number of cells available. 100 ng/mL heparin sodium salt Sigma H3149-250KU .
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Hydrogen peroxide, (15 M; H2O2; 1.07210.0250, Merck, Saint Louis, MO, USA) was used as a ROS generator, cysteine (0.4 mM; 100 μM 2-mercaptoethanol, 0.1 mM FGF4 (F2278, Sigma), and 1 μg/mL Heparin (H3149, Sigma). For TSC differentiation, cells were split to a 30% density without CellStart, FGF4 or heparin, and cultured at 20% or 2% oxygen for 5–10 days while changing media every other day.

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50 ng/mL human SCF (300-07, Peprotech) was included in the phase one of the culture (day 1-3) and withdrawn in the phase two of Heparin (H3149, Sigma-Aldrich, St. Louis, MO, USA), 5% newborn calf serum (N4762, Sigma-Aldrich, St. Louis, MO, USA), 0.2 g/mL bovine serum albumin (BSA) (A9418, Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-glutamine (G7513, Sigma-Aldrich, St. Louis, MO, USA), 100 g/mL Streptomycin, and #T4132), 1% penicillin/streptomycin, 10ng/ml heparin (Sigma, H3149), 10ug/ml insulin (Sigma, Cat. #I9278), 1ug/ml doxycycline. Peripheral blood mononuclear cells (PBMCs) were obtained from the University of Pennsylvania Human Immunology Core. 2020-10-13 · High Five™ cells (#B85502, Gibco) were cultured in Express Five SFM (#10486025, Gibco) supplemented with 18 mM L-glutamine (#25030081, Gibco), 10 U/mL heparin (#H3149, Sigma-Aldrich), and 0.25% penicillin-streptomycin. Insect cells were maintained in an incubator at 28°C. Method Details PBMC Isolation Endothelial cells were grown in “normal growth medium” (NGM): MCDB 131 (Caisson Labs) supplemented with 10% heat-inactivated FBS (F0926; Sigma), 25 mg/mL endothelial mitogen (BT-203, Biomedical Technologies), 2 U/mL heparin (H3149, Sigma), 1 μg/mL hydrocortisone (H0888, Sigma), 0.2 mmol/L ascorbic acid 2-phosphate (49752, Sigma), and 1% penicillin–streptomycin–glutamine (10378016 The heparin used in the study (catalog no.

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Chemical Compound, Drug, Heparin Sulfate, Sigma-Aldrich, Cat# H3149. Heparin sodium salt from porcine intestinal mucosa (Sigma-Aldrich, cat. no. H3149) ! CAUTION PFA is toxic. Perform all procedures in a fume hood. Hydrogel Sep 9, 2020 1% heparin (Sigma, H3149-100KU) and 15 μg ml−1 endothelial cell growth supplement (Merck, 324845)) for the first 5 days, and then cells H3149, Sigma) The cells grow as phase-bright, smooth spheres.

For TSC differentiation, cells were split to a 30% density without CellStart, FGF4 or heparin, and cultured at 20% or 2% oxygen for 5–10 days while changing media every other day. Cells were grown in DMEM-F12 containing 10% fetal bovine serum, 4 mmol/l glutamine (cat. no. 25030081), 1 ng/ml basic fibroblast growth factor (cat. no.